Binary vector pcambia-1300

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August undefined, 2024

http://www.biovector.net/product/1319.html Web作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。. 通过Google搜索"Biovector+您所需 …

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WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents inadvertent abortion

Nucleotide - Binary vector pCAMBIA-1300, complete sequence

WebNov 25, 2024 · In addition, we tested the pCAMBIA 1300-PtHCA 2-1 promoter-GUS binary expression vector, which contains the GUS gene ORF under the control of Populus … WebJul 24, 2013 · A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase ( hpt) marker gene. inch bar dunedin

pCambia1300 Plant Expression Vector (ab275754) Abcam

WebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of … WebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure … inadvertence meansWebSep 21, 2024 · A genetically engineered chimeric virus crTMV-CP-PLRV composed of the crucifer-infecting tobacco mosaic virus (crTMV) RNA and the potato leafroll virus (PLRV) coat protein (CP) was obtained by agroinfiltration of Nicotiana benthamiana with the binary vector pCambia-crTMV-CPPLRV. The significant levels of the chimeric virus enabled … inch barrel smith wesson ar 15 rilfe

"WebA twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase (hpt) marker gene. " - Binary vector pcambia-1300

Binary vector pcambia-1300

pCambia1300 Plant Expression Vector (ab275754) Abcam

WebSep 1, 2015 · The pCambia 2300 vector assumes stable genomic insertion and expression in plant species ( Hajdukiewicz et al., 1994) and the proper Gateway®-like function and … WebJul 1, 2024 · The pCAMBIA 1300 plasmid containing a GFP reporter gene driven by the Super promoter and harboring a Hygromycin resistance gene was used for transformation of somatic embryos. ... we transfected embryogenic calli with A. tumefaciens strain GV3101 containing a pCAMBIA 1300 binary vector, ...

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Web作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。. 通过Google搜索"Biovector+您所需资源名称",您一定能找到所需的资源和技术服务。. 如有需要请联系：. Dr Xu,Senior Scientist. 电邮 ... WebNov 25, 2024 · Agrobacterium tumefaciens strain GV3101 was transformed with the pBI121, pCAMBIA 1300, and recombinant pCAMBIA-PTHCA 2-1 Promoter-GUS binary vectors ( Supplementary Figure S1 ). The pBI121 …

WebJun 9, 2024 · In the first step, an EcoRI/KpnI fragment of ~ 2.4 kb was excised from the plasmid pKSMYMIV-A and cloned into the EcoRI/KpnI sites of binary vector pCAMBIA-1300 (Cambia) to produce the recombinant plasmid pCAM-MYMIV-AM. WebpCAMBIA1302. Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GFP genes. Sequence Author: Cambia. Open in …

WebThe cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants. WebBinary vector pCAMBIA-1300 NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, …

WebJun 19, 2007 · Biological Sciences Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of rice Akiko Sugio, Bing Yang, Tong Zhu, and Frank F. White Authors Info & …

WebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... inadvertent accordWebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields inch barrelWebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β … inadvertent associated wordsWebNov 9, 2015 · For genetic complementation, the full-length genomic DNA of the VPT1 fragment was amplified from wild type genomic DNA and cloned into the binary vector pCAMBIA-1300. To generate the VPT1VPT1 coding sequence without a stop codon, and GFP (35S:VPT1-GFP) were cloned into the pCAMBIA-1300. The tonoplast marker, … inadvertent administration meaningWebProduct name pCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for … inadvertent activationWebpCAMBIA2301 Agrobacterium binary vector for plant transformation, with kanamycin-resistance and GUS genes. Sequence Author: Cambia Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Plant Vectors More Plasmid Sets Home Plasmids Plant VectorspCAMBIA2301 Show … inch base cabinetWebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,... inch bathroom cabinet