WebThermo Scientific™ Accucore™ HILIC columns are recommended for the analysis of basic or neutral polar compounds. Catecholamines Column: Accucore HILIC, 2.6µm, 50 x 2.1mm Mobile Phase: 85:15 (v/v) acetonitrile / 100mm ammonium formate (pH 3.2) Flow Rate: 2mL/min Temperature: 40°C Detection: UV at 280nm Injection Volume: 5µL Backpressure: … WebHILIC is an alternative HPLC mode for separating polar compounds. • HILIC employs conventional polar stationary phases such as silica, amino, or cyano. • A typical mobile phase for HILIC includes water-miscible polar organic solvents such as acetonitrile, acetone, isopropanol, and methanol. •

Liquid Chromatography: LC Basics and Separation Techniques

WebMay 12, 2024 · Professor Alpert introduced a new concept in 1990: hydrophilicity chromatography (HILIC). This chromatographic method is used to separate strong polar and hydrophilic compounds, such as nucleosides and nucleotides, amino acids, sugars, etc. Besides, it uses polar stationary phase, polar mobile phase, and solutions with lower … WebApr 29, 2011 · Abstract. Hydrophilic interaction chromatography (HILIC) is valuable alternative to reversed-phase liquid chromatography separations of polar, weakly acidic or … date the 15th amendment was ratified

Stationary and mobile phases in hydrophilic interaction

WebHILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest. WebThis stationary phase readily and selectively retains polar compounds such as glycans. In addition, this sorbent possesses a weakly basic surface allowing the added potential to exploit ion exchange/repulsion properties. The eluent pH and ionic strength can impact retention on this HILIC phase and cannot be ignored. Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses … See more Any polar chromatographic surface can be used for HILIC separations. Even non-polar bonded silicas have been used with extremely high organic solvent composition, when the silica used for the chromatographic … See more A typical mobile phase for HILIC chromatography includes acetonitrile ("MeCN", also designated as "ACN") with a small amount of water. However, any aprotic solvent miscible with water (e.g. THF or dioxane) can be used. Alcohols can also be used, … See more The HILIC mode of separation is used extensively for separation of some biomolecules, organic and some inorganic molecules by … See more In 2008, Alpert coined the term, ERLIC (electrostatic repulsion hydrophilic interaction chromatography), for HILIC separations where an ionic column surface chemistry is used to repel a common ionic polar group on an analyte or within a set of … See more Ionic additives, such as ammonium acetate and ammonium formate, are usually used to control the mobile phase pH and ion strength. In HILIC they can also contribute to the polarity of the analyte, resulting in differential changes in retention. For extremely polar … See more With surface chemistries that are weakly ionic, the choice of pH can affect the ionic nature of the column chemistry. Properly adjusted, the pH can … See more For example, one could use a cation exchange (negatively charged) surface chemistry for ERLIC separations to reduce the influence on retention of anionic (negatively charged) … See more date that slavery ended